Glucose valve and other metabolite valves

摘要

Aspects of the invention relate to the design and construction of Metabolite Valves, such as Glucose Valves, that can be used to divert metabolites from endogenous pathways toward alternative pathways in a cell.

主权项

1. A method for redirecting glycolytic flux in a E. coli cell, the method comprising reducing expression of the phosphoenolpyruvate (PEP)-dependent glucose phosphotransferase system (PTS) in the cell and inhibiting phosphorylation of glucose by glucokinase within the cell, wherein the cell has increased expression of galactose permease (ga/P) and/or glucose facilitator protein (glf), wherein inhibiting phosphorylation of glucose by glucokinase within the cell comprises reducing expression of glucokinase in the cell, wherein reducing expression of glucokinase in the cell comprises recombinantly expressing in the cell an antisense RNA transcript that targets glucokinase, or replacing the promoter of glukokinase with a repressible promoter.