Materials and methods related to sodium/potassium adenosine triphosphase and cholesterol

摘要

This invention is based in part on the elucidation of new structural conformations and functions of the sodium/potassium adenosine triphosphate synthase (Na/K ATPase), and especially elucidation of new binding sites and interactions. The present invention provides practical applications of several surprising structural and functional relationships between Na/K ATPase and compounds which interact with Na/K ATPase. Disclosure of these structures and relationships provides insight and practical solutions to chemically affecting not only the Na/K ATPase interactions, but also regulators known to be upstream and downstream.

主权项

1. A method to affect cholesterol transport in a cell, comprising:
administering to the cell a composition of matter comprising an amino acid compound of 17 residues comprising at least ten consecutive amino acid residues of a cholesterol recognition/interaction amino acid sequence and consensus pattern (CRAC) sequence having SEQ ID NO:2, or conservative substitutions of the at least ten consecutive amino acid residues of SEQ ID NO:2, wherein the compound is capable of binding cholesterol and affecting sodium/potassium adenosine triphosphate synthase (Na/K ATPase) cholesterol-binding activity by antagonizing the CRAC domain on an α1 subunit of Na/K ATPase.