Methods and system for the automated determination of immunofluorescent foci using a cell-based immunofluorescence assay using synthetic calibration particles

摘要

The invention relates to a method for automated determination of immunofluorescent foci by means of an immunofluorescence assay using synthetic calibration particles, in addition to a system and kit for carrying out the method. In a preferred embodiment the method is characterized in that the immunofluorescent foci are gamma H2Ax foci.

主权项

1. Method for automated determination of cellular immunofluorescent foci by an immunofluorescence assay, comprising:
a. providing a mixture of cells to be analyzed and synthetic calibration particles, whereby the cells and particles are fixed to a solid phase, b. detecting the synthetic calibration particles, followed by calibrating and focusing of a fluorescent microscopy device and an automated evaluation system based on said detecting of the synthetic calibration particles, c. incubating said mixture with one or more antibodies that bind to a target substrate of the cells, d. detecting the antibodies bound to said substrates via said fluorescent microscopy device, and e. determining immunofluorescent foci from image data generated by said fluorescent microscopy device using said automated evaluation system.