Method for detection and/or analysis of yeast and mold in filterable liquids

摘要

This invention is a process for preparing a food or beverage sample containing yeast or mold cells for analytical testing. The food sample is prepared into the form of a filterable liquid, and then filtered using a glass microfiber filter. The filter containing the fungal cell retentate is then placed into a disruption vessel and bead beaten until the glass microfiber filter is completely disrupted into glass fibers in suspension. An aliquot can then be tested directly using melting curve analysis of PCR amplification product derived from the nucleic acids of the sample to detect the presence of the fungal cells from the sample.

主权项

1. A process for preparing a sample comprising fungal cells for analytical testing of the nucleic acids of said fungal cells, comprising the steps of:
(i) preparing said sample in the form of a filterable liquid; (ii) filtering said filterable liquid using a glass microfiber filter wherein said fungal cells from said sample form a retentate on said glass microfiber filter; (iii) transferring said glass microfiber filter to a vessel suitable for disrupting said glass microfiber filter and said fungal cells by mechanical means; (iv) simultaneously disrupting said fungal cells and said glass microfiber filter using mechanical means with a force sufficient to disrupt said glass microfiber filter into microscopic particles;wherein said microscopic particles of said disrupted glass microfiber filter are not removed from the nucleic acids of said fungal cells of said sample prior to analytical testing; and
(v) analytically testing the disrupted fungal cells and glass microfiber filter of step (iv) using amplification and melting curve analysis of PCR amplification product derived from the nucleic acids of the sample.