Scanning system and method for imaging and sequencing

摘要

A scanning detection system is provided in which emissions from locations in a flow cell are detected. In some embodiments, the system can include an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

主权项

1. A polynucleotide sequencing system, comprising:
a flow cell having at least one channel, a plurality of polynucleotides disposed in the at least one channel, at least a portion of the plurality of polynucleotides comprising a detectable label; a first irradiation source configured to generate a first excitation beam which irradiates a first group of polynucleotides of the plurality of polynucleotides thereby exciting at least one detectable label of at least one polynucleotide of the first group of polynucleotides such that the at least one detectable label of the at least one polynucleotide of the first group of polynucleotides emits an emission signal; a second irradiation source configured to generate a second excitation beam which irradiates a second group of polynucleotides of the plurality of polynucleotides thereby exciting at least one detectable label of at least one polynucleotide of the second group of polynucleotides such that the at least one detectable label of the at least one polynucleotide of the second group of polynucleotides emits an emission signal, the second group of polynucleotides being different from the first group of polynucleotides, the first and second excitation beams simultaneously irradiating the first and second groups of polynucleotides, respectively, wherein the first excitation beam does not substantially overlap the second excitation beam; a detection system having at least a first detector configured to detect the emission signal from the at least one detectable label of the at least one polynucleotide of the first group of polynucleotides and having at least a second detector configured to detect the emission signal from the at least one detectable label of the at least one polynucleotide of the second group of polynucleotides, the first and second detectors configured to detect distinct wavelengths or wavelength ranges relative to one another corresponding to distinct nucleotides; and a computer in operative communication with the detection system and configured to collect and process the detectable emissions from the first and second groups of polynucleotides.