METHOD FOR PRODUCING ANTIRABIES VACCINE

摘要

The invention relates to veterinary microbiology and biotechnology, and can be used in the development of means of specific prophylaxis, in particular for producing a vaccine against rabies in animals. A method for producing an antirabies vaccine for animals is proposed, which comprises preparation of an inoculum from a rabies virus strain, inoculation with the inoculum of a transferable cell culture, cultivation of the rabies virus, harvesting a virus-containing suspension followed by inactivation and preparation of the end product. The inactivation is conducted by adding ethanol to the virus-containing suspension in a final concentration of 18-20%, maintenance at a temperature of 36-37°C for 20-22 hours while stirring continuously, followed by the addition of 4-6% aluminium hydroxide in a ratio to the reaction mixture of 1:(4.5-5.0).