Medio libre de sangre solido y liquido para el cultivo de p. salmonis; metodo de prepararción de dicho medio; metodo para simplificar el aislamiento de células de p salmonis; metodo para evaluar la aparición de resistencia a los antibioticos de p. salmonis que comprende el caldo antes mencionado.

摘要

The present invention relates to new contamination resistant artificial media for the routine cultivation of P. salmonis. In particular, it discloses first a liquid media based named Austral-SRS Broth that extensively require iron and sodium chloride; and, second, a blood-free agar media comprising tryptone soy with either ferric or ferrous salts (Austral- TSFe) agar or the use of hemoglobin (Austral-TSHem) as a source of iron in the agar. Also disclosed is a method for their preparation as well as their application in the development of antigens and low cost vaccines for protection against Piscirickettsiosis and the use of the medias for a kit that evaluate the antibiotic resistance appearance in different strain of P. salmonis isolated from the salmon industry.