发明名称 |
HIGH THROUGHPUT METHOD OF SCREENING A POPULATION FOR MEMBERS COMPRISING MUTATION(S) IN A TARGET SEQUENCE |
摘要 |
The present invention provides high-throughput methods of screening for members of a population comprising mutation(s) in one or more target sequence(s). The methods may comprise the steps of: pooling genomic DNA isolated from each member of said population; amplifying the one or more target sequence(s) in the pooled genomic DNA; pooling the amplification products of step (b) to create a library of amplification products; sequencing the amplified products by pair-end sequencing to produce paired-end reads for each sequencing reaction or obtaining paired-end sequence reads for the amplified products; merging the paired-end reads into composite read(s); mapping the composite read(s) to reference sequence(s) to identify mutation(s) in the one or more target sequence(s); and identifying member(s) of the population comprising one or more of the identified mutations in the target sequence(s). The invention further provides kits for use with the methods. |
申请公布号 |
WO2014134729(A1) |
申请公布日期 |
2014.09.12 |
申请号 |
WO2014CA50177 |
申请日期 |
2014.03.06 |
申请人 |
VINELAND RESEARCH AND INNOVATION CENTRE |
发明人 |
BANKS, TRAVIS WILFRED;SOMERS, DARYL JOHN |
分类号 |
C12Q1/68;C07H21/04;C12N15/00;C12N15/29;C40B30/04;G06F19/22 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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