| 摘要 |
A fluorescence detection device (1) is provided with the following: an excitation light source (10); a phase-modulating spatial light modulator (20) that can phase-modulate excitation light (L1), yielding phase-modulated light (L2); focusing optics (30) that are provided after the spatial light modulator (20) and focus the phase-modulated light (L2) onto a sample (90); a sample stage (92) onto which said sample (90) is placed; a fluorescence detector (40) that detects fluorescence (L3) produced as a result of the phase-modulated light (L2) being focused onto the sample (90); a control unit (21) that displays a first CGH on the spatial light modulator (20); and a correction unit (22) that corrects said first CGH. Said correction unit (22) is provided with the following: a detector-specific sensitivity-information storage unit (222) that, in advance, acquires and stores sensitivity information specific to the fluorescence detector (40) per detection position; and a second-hologram generation unit (223) that generates a second CGH by correcting the first CGH on the basis of the intensity of the fluorescence (L3) and the aforementioned sensitivity information. The control unit (21) displays said second CGH on the spatial light modulator (20). |
