EVALUATION METHOD AND EVALUATION KIT OF SUBSTANCE, SCREENING METHOD OF SUBSTANCE, AND DETECTION METHOD AND DETECTION KIT FOR PRESENCE OF ONSET OF DIABETIC NEPHROPATHY OR FUTURE ONSET RISK

摘要

PROBLEM TO BE SOLVED: To identify a new biomarker reflecting future onset risk of diabetic nephropathy and provide a series of techniques utilizing the biomarker.SOLUTION: An animal having the onset of diabetic nephropathy or an animal with future onset risk is allowed to take a test substance; concentration of at least one of 8 types of marker substances in a biological fluid of the animal is compared with a reference value, and an improvement effect of diabetic nephropathy or a reduction effect of future onset risk that the test substance has is evaluated. The marker substance is captured on a carrier on which a substance having affinity to the marker substance is immobilized, and concentration of the marker substance in the biological fluid can be also calculated. There are also provided a screening method of a substance utilizing the evaluation method, and a determination method of the onset of diabetic nephropathy or future onset risk by using the marker substance as an index.