发明名称 |
ENZYME METHOD |
摘要 |
The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore. |
申请公布号 |
US2014255921(A1) |
申请公布日期 |
2014.09.11 |
申请号 |
US201214351038 |
申请日期 |
2012.10.18 |
申请人 |
Oxford Nanopore Technologies Limited |
发明人 |
Moysey Ruth;Heron Andrew John |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A method of characterising a target polynucleotide, comprising:
(a) contacting the target polynucleotide with a transmembrane pore and a Hel308 helicase such that the helicase controls the movement of the target polynucleotide through the pore and nucleotides in the target polynucleotide interact with the pore; and (b) measuring one or more characteristics of the target polynucleotide during one or more interactions and thereby characterising the target polynucleotide. |
地址 |
Oxford GB |