发明名称 |
GENE EXPRESSION CASSETTE AND A TRANSFORMANT, AND A METHOD FOR MANUFACTURING 2-DEOXY-SCYLLO-INOSOSE AND A METHOD FOR PURIFYING 2-DEOXY-SCYLLO-INOSOSE USING SAID TRANSFORMANT |
摘要 |
A transformant is prepared to insert at least a gene expression cassette comprising a gene involved in the synthesis of 2-deoxy-scyllo-inosose into E. coli as host cells. A 2-deoxy-scyllo-inosose is synthesized from D-glucose, oligosaccharide, polysaccharide, starch and rice bran, using the transformant. A culture solution containing the 2-deoxy-scyllo-inosose is treated with a mixed bed or double bed type column comprising a hydrogen form of strong acidic cation exchange resin and an organic ion form of basic anion exchange resin. The 2-deoxy-scyllo-inosose as purified is reacted with trimethoxymethane to convert into 2-deoxy-scyllo-inosose dimethylketal, and the dimethylketal is crystallized and purified. Then, DOI is highly purified through hydrolyzing the dimethylketal in the presence of acid. |
申请公布号 |
US2014256960(A1) |
申请公布日期 |
2014.09.11 |
申请号 |
US201414178496 |
申请日期 |
2014.02.12 |
申请人 |
MITSUI CHEMICALS, INC. ;NIIGATA BIO-RESEARCH PARK, INC. |
发明人 |
TAKAGI Masamichi;Kogure Takahisa;Wakisaka Naoki;Takaku Hiroaki;Ajisaka Katsumi;Miyazaki Tatsuo;Hirayama Masao |
分类号 |
C12P17/06;C07D309/30 |
主分类号 |
C12P17/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
Tokyo JP |