| 发明名称 |
PROKARYOTE-BASED CELL-FREE SYSTEM FOR THE SYNTHESIS OF GLYCOPROTEINS |
| 摘要 |
The present invention is directed to a cell-free system for producing a glycosylated protein. This system comprises an isolated oligosaccharyltransferase capable of transferring a glycan from a lipid carrier molecule to a glycoprotein target, one or more isolated glycans, where each glycan is linked to a lipid carrier molecule, and a glycoprotein target comprising one or more glycan acceptor amino acid residues or a nucleic acid molecule encoding said glycoprotein target. The present invention further relates to kits and methods for producing a glycosylated protein in this cell-free system. |
| 申请公布号 |
US2014255987(A1) |
申请公布日期 |
2014.09.11 |
| 申请号 |
US201214356258 |
申请日期 |
2012.11.05 |
| 申请人 |
CORNELL UNIVERSITY |
发明人 |
Delisa Matthew |
| 分类号 |
C12P21/00;C12N9/10 |
主分类号 |
C12P21/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A cell-free system for producing a glycosylated protein comprising:
an isolated oligosaccharyltransferase capable of transferring a glycan from a lipid carrier molecule to a glycoprotein target; one or more isolated glycans, wherein each glycan is linked to a lipid carrier molecule; and a glycoprotein target comprising one or more glycan acceptor amino acid residue, or a nucleic acid molecule encoding said glycoprotein target. |
| 地址 |
Ithaca NY US |
