Probes for identifying cancer-specific antigens

摘要

This invention relates to the diagnosis of cancer, and in particular to the identification and detection of cancer-specific antigens. The invention provides antibody probes and methods for using the probes 5 for detection and purification of cancer-specific anLigens, and in the preparation of vaccine compositions. Antibodies against cancer specific antigens are also disclosed and claimed. 32173191 (GHMAtlter) P4D46O.AU.3 Detection of cancer-associated antigens using ASC-probes Draining (Interactive) Lymph node lymph nodes Cancer cells lymphocytes Solubilised'in Activated B SDS-PAGE cells put into sample buffer tissue culture Cancer Antibodies secreted into culture medium used to probe Western blots Proteins are transferred to nitrocellulose for --- Cancer proteins Western blot - Cancer specific ~ separated by analysis proteins recognised - molecular weight by cancer specific on SDS-PAGE antibodies SDS-PAGE Western blot e Cancer specific proteins identified with the cancer specific antibodies are purified and further characterised using both immunochemical and standard biochemical and biophysical techniques. Figure 1