TWO-PHOTON ENDOSCOPIC SCANNING ASSEMBLY FOR INFLAMMATORY DISEASE DETECTION

摘要

An endscopic imaging device is described that achieves longitudinal axis (z-axis) scanning into a tissue or sample, using a piezoelectric microactuator. In some configurations, additional lateral (xy-plane) scanning is also achieved, to allow for the creation of full three-dimensional imaging, ex vivo or in vivo. The techniques may be used to image and diagnosis allergic rhinitis and eosinophilic esophagitis in tissue.

主权项

1. A method of detecting a biomarker within a 3-dimensional volume of a sample, the method comprising:
providing an endoscopic assembly for producing a output laser energy, the endoscopic assembly comprising an xy scanning stage and an actuator stage for z-axis scanning within the sample; the xy scanning stage scanning the output laser beam over a planar scan area of the sample; the actuator stage scanning the output laser beam over a depth range of the sample, where the depth range and the planar scan area form the 3-dimensional volume, driving the actuator stage using a piezoelectric stage and mechanically amplifying a resulting displacement of the piezoelectric stage to scan the two-photon output beam over the entire depth range; sampling a plurality of points within the sample by collecting fluorescence resulting from interaction of the output laser beam and the sample at each of the points; and detecting the biomarker from the fluorescence collected from each the plurality of points.