VIRUS-LIKE PARTICLES AND PROCESS FOR PREPARING SAME

摘要

An in vitro process of preparing virus-like particles (VLPs) from recombinant papaya mosaic virus coat protein and ssRNA, which allows for large scale production of VLPs in high yields, is provided. Also provided are VLPs comprising ssRNA prepared by the in vitro process. The VLPs can be used as adjuvants and when fused to an antigen, as vaccines. The use of the VLPs for stimulation of the innate immune response is also provided.

主权项

1. An in vitro process for preparing virus-like particles (VLPs) comprising the steps of:
a) combining recombinant potexvirus coat protein and ssRNA at a protein:RNA ratio of between about 1:1 and 50:1 by weight, at a pH between about 6.0 and about 9.0, and a temperature between about 2° C. and about 37° C., for a time sufficient to allow assembly of VLPs; b) treating the VLPs with nuclease to remove any RNA protruding from the particles, and c) separating the VLPs from other process components.