ULTRAFAST SEQUENCING OF BIOLOGICAL POLYMERS USING A LABELED NANOPORE

摘要

Methods and systems for sequencing a biological molecule or polymer, e.g., a nucleic acid, are provided. One or more donor labels, which are attached to a pore or nanopore, may be illuminated or otherwise excited. A polymer having a monomer labeled with one or more acceptor labels, may be translocated through the pore. Either before, after or while the labeled monomer of the polymer passes through, exits or enters the pore, energy may be transferred from the excited donor label to the acceptor label of the monomer. As a result of the energy transfer, the acceptor label emits energy, and the emitted energy is detected in order to identify the labeled monomer of the translocated polymer and to thereby sequence the polymer.

主权项

1. A method for sequencing a polymer comprising:
exciting a donor label attached to a nanopore; translocating a polymer through the nanopore, wherein the polymer comprises a monomer labeled with an acceptor label; transferring energy from the excited donor label to the acceptor label of the monomer after the labeled monomer passes through and exits the nanopore; detecting the energy emitted by the acceptor label as a result of the energy transfer, wherein the energy emitted by the acceptor label is associated with a single monomer; and deducing the polymer sequence based on the detection of the emitted energy from the acceptor label and identification of the monomer.