ENZYME FUNCTION MODIFICATION METHOD AND ENZYME VARIANT THEREOF

摘要

An object of the present invention is to provide a method for converting the coenzyme dependency of enzymes of the medium-chain dehydrogenase/reductase (MDR) family. A further object of the present invention is to provide enzyme variants of the MDR family whose coenzyme dependency is converted by the conversion method and a method for enzymatically producing optically active alcohols using the enzymes. The present inventors developed a novel enzyme conversion method for converting the coenzyme dependency of enzymes of the MDR family, rationally designed enzyme variants that are altered by the enzyme conversion method to be able to use NADPH as a coenzyme from a useful enzyme of the MDR family that uses NADH as a coenzyme, and actually provide variants having such an ability.

主权项

1. A protein of medium-chain dehydrogenase/reductase family, containing at least one of the following amino acid residues (a) to (d):
(a) Ala or Ser at a position conformationally equivalent to Asp-201 of SEQ ID NO:1; (b) Arg at a position conformationally equivalent to Lys-202 of SEQ ID NO:1; (c) Ser at a position conformationally equivalent to Lys-203 of SEQ ID NO:1; and (d) Lys at a position conformationally equivalent to Ala-206 of SEQ ID NO:1.