EXTRACTION BUFFER AND METHOD FOR ISOLATING HIGH-QUALITY RNA FROM CELLS EXPOSED TO METAL CHLORIDE SOLUTIONS AND CLAY MINERAL SUSPENSIONS

摘要

An RNA extraction buffer, an RNA extraction method, and an RNA extraction kit are described which enable functional, rapid, efficient, and high-quality RNA isolation from samples containing high concentrations of aqueous metal cations, clays, silica, or silicate minerals

主权项

1. An extraction buffer for isolating RNA comprising:
a sodium phosphate buffer with a pH of about 4.5 to about 6.0; about 1.0 M to about 2.0 M NaCl; about 0.2% hexadecyltrimethylammonium bromide (CTAB); and a chelating agent.