ALKALINE ACTIVATION FOR IMMOBILIZATION OF DNA TAGGANTS

摘要

The invention provides methods for stably binding and immobilizing deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate. The alkaline conditions are produced by mixing the deoxyribonucleic acid with an alkaline solution having a pH of about 9.0 or higher, and contacting the deoxyribonucleic acid to the substrate. The immobilized DNA can be used as a taggant and can be used in combination with other detectable taggants, such as optical reporters. Methods for authentication of a DNA marked object are also provided.

主权项

1. A method of binding a deoxyribonucleic acid to a substrate, the method comprising:
exposing the deoxyribonucleic acid to an alkaline pH, and contacting the alkaline exposed deoxyribonucleic acid to the substrate.