UNIQUE BUFFERING SYSTEM FOR CELL CULTURE MEDIA AND GAMETE AND EMBRYO CULTURE MEDIA AND METHODS

摘要

The invention relates to compositions and methods for continuous stabilization of pH of cell culture medium notwithstanding change from one distinct environment to another environment. The methods include providing a buffering system that includes a sodium bicarbonate buffer and at least two zwitterionic buffers having concentrations significantly reduced as compared to conventionally used media buffers utilizing a single zwitterionic buffer.

主权项

1. A method for continuous stabilization of pH of cell culture medium notwithstanding change from the first environment conditions to the second environment conditions, comprising providing a buffering system, the buffering system comprising sodium bicarbonate at a concentration from 4 to 30 mM, and at least two zwitterionic buffers at a concentration from 1 to 50 mM,
wherein the pH is 7.0-7.6, and wherein the first and the second environment conditions are distinct.