| 主权项 |
1. A compound of general formula IIcwherein each of R1, R2, R5, and R6 is the same or different and is independently selected from the group consisting of an aliphatic, heteroaliphatic, sulfoalkyl group, heteroaliphatic with terminal SO3, a PEG group P-L-Z where P is selected from an ethylene glycol group, a diethylene glycol group, and a (poly)ethylene glycol group where the (poly)ethylene glycol group is (CH2CH2O)s where s is an integer from 3-6 inclusive, a sulfonamide group -L-SO2NH-P-L-Z, and a carboxamide group -L-CONH-P-L-Z;
each of R7 and R8 is the same or different and is independently selected from the group consisting of H, SO3, a PEG group P-L-Z where P is selected from an ethylene glycol group, a diethylene glycol group, and a (poly)ethylene glycol group where the (poly)ethylene glycol group is (CH2CH2O)s where s is an integer from 3-6 inclusive, a sulfonamide group —SO2NH-P-L-Z, and a carboxamide group —CONH-P-L-Z; each of R9 and R10 is the same or different and is independently selected from the group consisting of alkyl, sulfoalkyl, La-Z, and La-X; where La is selected from the group consisting of a divalent linear (—(CH2)o—, o=0 to 15), crossed, or cyclic alkane group that can be substituted by at least one atom selected from substituted nitrogen and/or sulfur; where L is selected from the group consisting of a divalent linear (—(CH2)o—, o=0 to 15), crossed, or cyclic alkane group that can be substituted by at least one atom selected from the group consisting of oxygen, substituted nitrogen, and/or sulfur; where Z is selected from the group consisting of H, CH3, an alkyl, a heteroalkyl, NH2, —COO−, —COOH, —COSH, CO—NH—NH2, —COF, —COCl, —COBr, —COI, —COO-Su (succinimidyl/sulfosuccinimidyl), —COO-STP (4-sulfo-2,3,5,6-tetrafluorophenyl), —COO-TFP (2,3,5,6-tetrafluorophenyl), —COO-benzotriazole, —CO-benzotriazole, —CONR′—CO—CH2—I, —CONR′R″, —CONR′-biomolecule, —CONR′-L-COO−, —CONR′-L-COOH, —CONR′-L-COO-Su, —CONR′-L-COO-STP, —CONR′-L-COO-TFP, —CONR′-L-CONR″2, —CONR′-L-CO-biomolecule, —CONK′-L-CO—NH—NH2, —CONR′-L-OH, —CONR′-L-O-phosphoramidite, —CONR′-L-CHO, —CONR′-L-maleimide, and —CONR′-L-NH—CO—CH2—I; each of R′ and R″ is independently selected from the group consisting of H, aliphatic group, and heteroaliphatic group, and the biomolecule is a protein, antibody, nucleotide, oligonucleotide, biotin, or hapten; X is selected from the group consisting of —OH, —SH, —NH2, —NH—NH2, —F, —Cl, —Br, I, —NHS (hydroxysuccinimidyl/sulfosuccinimidyl), —O-TFP (2,3,5,6-tetrafluorophenoxy), —O-STP (4-sulfo-2,3,5,6-tetrafluorophenoxy), —O-benzotriazole, -benzotriazole, —NR-L-OH, —NR-L-O-phosphoramidite, —NR-L-SH, —NR-L-NH2, —NR-L-NH—NH2, —NR-L-CO2H, —NR-L-CO—NHS, —NR-L-CO-STP, —NR-L-CO-TFP, —NR-L-CO-benzotriazole, —NR-L-CHO, —NR-L-maleimide, and —NR-L-NH—CO—CH2—I, where R is —H or an aliphatic or heteroaliphatic group; Kat is a number of Na+, K+, Ca2+, ammonia, or other cation(s) needed to compensate the negative charge brought by the cyanine; and n is an integer from 1 to 3 inclusive; with the proviso that at least one of R1, R2, R5, R6, R7, and R8 contains a PEG group. |
