Methods and Apparatus for Decomposing Tandem Mass Spectra Generated by All-Ions Fragmentation

摘要

A method for tandem mass spectrometry of a plurality of eluting compounds comprises: (a) performing, during a time period, the steps of: ionizing the plurality of eluting compounds to generate a plurality of precursor ion species; introducing the plurality of precursor ions into a fragmentation cell operated at constant fragmentation energy so as to generate a plurality of product-ion species from at least a portion of the precursor ion species; and generating a mass spectrum of the plurality of product-ion species; and (b) recognizing matches between certain of the product ion species generated during the time period based on correlations between elution profiles of the product ion species.

主权项

1. A method for acquiring and interpreting tandem mass spectra of a plurality of compounds that are introduced into a mass spectrometer from a chromatograph, said method comprising:
(a) repeatedly performing, during a time period, the steps of:
(a1) ionizing the plurality of compounds as they elute from the chromatograph so as to generate a plurality of precursor ion species therefrom using an ion source of the mass spectrometer;(a2) introducing the plurality of precursor ions into a fragmentation cell of the mass spectrometer operated at constant fragmentation energy so as to generate a plurality of product-ion species from all or a portion of each of the plurality of precursor ion species; and(a3) generating a mass spectrum of the plurality of product-ion species; and (b) recognizing matches between certain of the product ion species generated during the time period based on correlations between elution profiles of the product ion species determined from the plurality of generated mass spectra.