RAPID QUANTIFICATION OF BIOMOLECULES IN A SELECTIVELY FUNCTIONALIZED NANOFLUIDIC BIOSENSOR AND METHOD THEREOF

摘要

A method and device for the rapid quantification of biomolecules (320) present in a nanochannel (210) is claimed. In particular, the present invention relates to a novel concept of liquid actuation and selectively functionalized surfaces in a nanochannel that create a concentration gradient of transitory immobilized biomolecules (340) across the nanochannel. The present concept enables the quantification of biomolecular interactions of interest (320).

主权项

1. A biosensor for detecting and quantifying fluorescently-labeled biomolecules; said biosensor comprising a nanochannel defined between two substrates and containing one or several selectively functionalized areas on which are immobilized biomarkers, said nanochannel furthermore being defined by a lateral input aperture and a lateral output aperture, said input aperture being adapted to let a solution containing biomolecules to enter said nanochannel and said output aperture adapted to drive said solution through said nanochannel by capillarity.