Eukaryotic amadoriase, gene and recombinant DNA for the eukaryotic amadoriase, and process for production of the eukaryotic amadoriase

摘要

Disclosed are: a eukaryotic amadoriase which is prepared by introducing a mutation into DNA encoding a eukaryotic amadoriase derived from a microorganism belonging to the genus Coniochaeta or Eupenicillium so as to introduce a substitution into a specific amino acid residue in the eukaryotic amadoriase, thereby overcoming the defect associated with thermal stability; a gene or recombinant DNA for the eukaryotic amadoriase; and a process for production of a eukaryotic amadoriase having excellent thermal stability.

主权项

1. An eukaryotic amadoriase having the amino acid sequence of SEQ ID NO: 2, with the exception of:
(a) replacement of glycine at position 184 of SEQ ID NO: 2 by aspartic acid; (b) replacement of asparagine at position 272 of SEQ ID NO: 2 by aspartic acid; and (c) replacement of histidine at position 388 of SEQ ID NO: 2 by tyrosine; wherein said eukaryotic amadoriase retains at least 50% residual activity after a heat treatment at 50° C. and pH 8.0 for 30 minutes.