发明名称 |
Methods and compositions relating to isocyanate conjugates |
摘要 |
Compositions are provided according to embodiments of the present invention which include an isolated antibody or antigen binding antibody fragment characterized by binding specificity for a conjugate which is a reaction product of a protein moiety and an isocyanate moiety. Methods of detecting diisocyanate-protein conjugates in a sample are provided according to embodiments of the present invention which include contacting a sample with one or more isolated antibodies or antigen binding antibody fragments characterized by binding specificity for corresponding diisocyanate-protein conjugate antigens. |
申请公布号 |
US8828667(B2) |
申请公布日期 |
2014.09.09 |
申请号 |
US200912577241 |
申请日期 |
2009.10.12 |
申请人 |
The United States of America, as represented by the Secretary, Department of Health and Human Services |
发明人 |
Siegel Paul D.;Ruwona Tinashe Blessing;Beezhold Donald H.;Johnson Victor;Schmechel Detlef |
分类号 |
G01N33/53;C07K16/44 |
主分类号 |
G01N33/53 |
代理机构 |
Gifford, Krass, Sprinkle, Anderson & Citkowski, P.C. |
代理人 |
Gifford, Krass, Sprinkle, Anderson & Citkowski, P.C. |
主权项 |
1. A method of detecting diisocyanate-protein conjugates in a sample, comprising:
a) contacting a sample with a first isolated monoclonal antibody or antigen binding fragment thereof, wherein the first isolated monoclonal antibody or antigen binding fragment thereof binds to a 2,4-toluene diisocyanate moiety bound to a protein through a urea, urethane, carbamate or thiocarbamate linkage; and b) detecting binding of the first isolated monoclonal antibody or antigen binding fragment thereof with the 2,4-toluene diisocyanate moiety bound to a protein through a urea, urethane, carbamate or thiocarbamate linkage, and c) contacting the sample with a second monoclonal antibody or antigen binding fragment thereof wherein the second monoclonal antibody or antigen binding fragment thereof binds to 2,6-toluene diisocyanate moiety bound to a protein through a urea, urethane, carbamate or thiocarbamate linkage; and d) detecting binding of the second isolated monoclonal antibody or antigen binding fragment thereof with the 2,6-toluene diisocyanate moiety bound to a protein through a urea, urethane, carbamate or thiocarbamate linkage. |
地址 |
Washington DC US |