Method to enhance an immune response of nucleic acid vaccination

摘要

A composition comprising liposomes associated with a nucleic acid operatively encoding an antigenic protein and with an assistor protein, wherein the assistor protein shares at least one epitope with the antigenic protein, and wherein the nucleic acid and said assistor protein are associated with the same liposomes is described. The composition provides an improved immune response compared to mixtures of liposomes some of which are associated with the nucleic acid and some of which are associated with the assistor protein.

主权项

1. A composition for generating an immune response in a mammal, wherein said composition comprises vesicles formed of amphiphilic components, wherein said vesicles are associated with a nucleic acid and an assistor protein, wherein the nucleic acid operatively encodes an antigenic protein or portion thereof which shares at least one epitope with the assistor protein, wherein the vesicles have an average diameter in the range of 100-2000 nm, wherein the nucleic acid encoding the antigenic protein, and the assistor protein, are associated with the same vesicles; wherein the nucleic acid is entrapped in the intravesicular space of the vesicles and the assistor protein is displayed on the surface of the same vesicle, whereby the nucleic acid and assistor protein will be co-delivered by the vesicles to a cell.