Method and kit for detecting biological signal of three-dimensional cell culture material

摘要

Disclosed is a method for detecting a biological signal of a three-dimensional cell culture construct. The method for detecting a biological signal of a three-dimensional cell culture construct includes: providing a three-dimensional cell culture construct that contains at least two cell layers laminated to each other and a sensor particle capable of detecting a biological signal; and observing the sensor particle optically. Preferably, the three-dimensional cell culture construct contains an extracellular matrix including a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence, or a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.

主权项

1. A method for detecting a biological signal of a construct that is produced by a layer-by-layer (LBL) method, comprising:
providing a construct that is produced by an LBL method and contains at least two cell layers, an extracellular matrix, and a sensor particle capable of detecting a biological signal; and observing the sensor particle optically, wherein the construct is provided so that the extracellular matrix is formed between the cell layers, thereby laminating the cell layers to one another, and wherein the construct is provided so that the sensor particle is placed in the extracellular matrix between the cell layers.