Peptides, devices, and methods for the detection of ehrlichia antibodies

摘要

The invention provides compositions (e.g., peptide compositions) useful for the detection of antibodies that bind to Ehrlichia antigens. The peptide compositions comprise polypeptide sequences based on an immunogenic fragment of the Ehrlichia Outer Membrane Protein 1 (OMP-1) protein. The invention also provides devices, methods, and kits comprising such peptide compositions and useful for the detection of antibodies that bind to Ehrlichia antigens and the diagnosis of monocytic ehrlichiosis.

主权项

1. A mixture of isolated peptides, wherein each isolated peptide comprises the sequence of SEQ ID NO: 59,(SEQ ID NO: 59)F-S-A-K-Xs-X6-X7-A-E-T-XII-X12-T-F-G-X16-X17-X18-X19-X20-D-G-A-X24-X25-X26-X27-N-X29-V-X31-N-X33-F-T-I-S-Nwherein X5 is an amino acid selected from the group consisting of E and Q, X6 is an amino acid selected from the group consisting of E and Q, X7 is any amino acid, X11 is an amino acid selected from the group consisting of K and R, X12 is any amino acid, X16 is an amino acid selected from the group consisting of L and I, X17 is any amino acid, X18 is an amino acid selected from the group consisting of R and K, X19 is an amino acid selected from the group consisting of Q and N, X20 is an amino acid selected from the group consisting of Y and T, X24 is any amino acid, X25 is an amino acid selected from the group consisting of I and L, X26 is any amino acid, X27 is an amino acid selected from the group consisting of D and E, X29 is an amino acid selected from the group consisting of E and Q, X31 is an amino acid selected from the group consisting of E and Q, and X33 is an amino acid selected from the group consisting of K and R; and wherein any additional peptide sequence N-terminal or C-terminal to the sequence of SEQ ID NO: 59 is a non-native sequence, wherein each isolated peptide is conjugated to a ligand or wherein each isolated peptide is immobilized to a solid support.