Massively parallel 2-dimensional capillary electrophoresis

摘要

This invention discloses a highly efficient method, system and apparatus for nucleic acid analysis, including sequencing (both automated re-sequencing and de-novo sequencing). The system is capable of sequencing DNA sizes ranging from fragments to mammalian size genomes having mouse draft quality at a much reduced cost. The system comprises a massive parallel capillary electrophoretic separation using two-dimensional monolith multi-capillary arrays (2D-MMCA). Sequence identification can be performed using fluorescent or otherwise labeled dideoxynucleotide-terminated DNA extension product generated by gel matrix-, or beads-, or substrate tethered-, or otherwise immobilized colonies of single template molecules. Cost reduction is a significant advantage over currently known methods because of: (i) using massively parallel sub-nanoliter volume reactions; and (ii) employing 2D-MMCAs that increase the throughput of the CE separation and detection by at least two orders of magnitude compared to the commercial high-throughput DNA machines.

主权项

1. A system for nucleic acid analysis comprising
a) a plurality of pads disposed on a solid substrate, at least a portion of said pads comprising nucleic acid, at least a portion of said nucleic acid comprising a marker, said substrate comprising an electrically conductive element; b) a plurality of capillaries, each said capillary comprising first and second ends, said first end alignable with a pad in said plurality of pads without contacting said pad, said second end alignable with c) a photodetector configured to detect said marker when said nucleic acid is in said capillaries.