Salivary mRNA Profiling, Biomarkers and Related Methods and Kits of Parts

摘要

A method to detect a biomarker in saliva wherein the biomarker is an extracellular mRNA, comprises detecting the extracellular mRNA in the cell-free saliva; transcriptome analysis of saliva comprises detecting a transcriptome pattern in the cell-free saliva; a method to detect genetic alterations in an organ or in a gene in the organ by analyzing saliva, comprises detecting a transcriptome pattern and/or the mRNA profiling of the gene in cell-free saliva; a method to diagnose an oral or systemic pathology disease or disorder in a subject, comprises: detecting profile of a biomarker associated with the pathology disease or disorder, in particular mRNA and/or protein, in cell-free saliva and/or serum; kits comprising identifier for at least one biomarker for performing at least one of the methods; and use of salivary biomarker salivary and/or serum mRNAs as biomarkers for oral and/or systemic pathology, disease or disorder.

主权项

1. A method for diagnosing a pathology in a subject comprising the steps of:
measuring the amount of IL8 protein in a saliva sample of a subject; comparing the amount of IL8 protein in the saliva sample of the subject with the amount of IL8 protein in a saliva sample of a control subject known to not have the pathology; determining that the amount of IL8 protein in the saliva sample of the subject is elevated relative to the amount of IL8 protein in the saliva sample of the control subject known to not have the pathology; and diagnosing the pathology in the subject when the amount of IL8 protein in the saliva sample of the subject is elevated relative to amount of IL8 protein in the saliva sample of the control subject known to not have the pathology.