METHOD AND SYSTEM FOR ABO ANTIBODY DETECTION AND CHARACTERIZATION

摘要

The present application discloses a system and method for ABO antibody detection and characterization that can provide an alternative means for assessment and management of ABO-incompatible and ABO-compatible transplants. The method and system comprises determining an anti-ABO blood group antigen subtype antibody profile of a subject using a biological sample from the subject. The method and system can be used to evaluate the suitability of a donor blood or tissue product for a recipient subject by comparing the determined anti-ABO antigen subtype antibody profile of the recipient subject with the ABO histo-blood group or ABO histo-blood subgroup of a donor blood or tissue product. In order to define the subject's ABO histo-blood subgroup, the determined antibody profile is compared to known ABO histo-blood group antigen subtype profiles and/or known anti-ABO antigen subtype antibody profiles for ABO histo-blood subgroups to identify the ABO histo-blood subgroup of the subject. Profiles can be established by applying a sample to an array of surface-bound ABO antigens selected from the group of type I to type VI antigens of each blood group A, B or H.

主权项

1. A method of identifying the ABO histo-blood subtype antibody profile of a subject comprising:
(a) identifying the presence or absence of antibodies specific for at least one ABO histo-blood group antigen subtype in a biological sample from said subject; and (b) using the information from step (a) to generate the ABO antigen subtype antibody profile of the subject, wherein step (a) optionally includes quantifying the amount of the at least one anti-ABO histo-blood group antigen subtype antibody in the biological sample.