Whole Slide Fluorescence Scanner

摘要

A system for creating a contiguous digital image of a fluorescence microscope sample. In an embodiment, the system comprises a both a macro camera and a time delay integration (TDI) line scan camera. The system may also comprise a motorized stage, an illumination module, a light source, an excitation filter, an objective lens, an emission filter, and at least one processor configured to assemble a plurality of digital images of portions of the fluorescence microscope sample, generated by the TDI line scan camera, into a contiguous digital image of at least a portion of the fluorescence microscope sample.

主权项

1. A system for creating a contiguous digital image of a portion of a fluorescence microscope sample, the system comprising:
a motorized stage configured to support a fluorescence microscope sample and move the fluorescence microscope sample at a substantially constant velocity; an illumination module configured to apply oblique illumination to the fluorescence microscope sample on the motorized stage; a macro camera configured to capture an image of the fluorescence microscope sample on the motorized stage during oblique illumination; a light source configured to provide excitation light to a portion of the fluorescence microscope sample via an illumination path while the fluorescence microscope sample on the motorized stage is moving at substantially constant velocity; an excitation filter in the illumination path; a time delay integration (TDI) line scan camera comprising a plurality of linear sensor arrays configured to generate a digital image of a portion of the fluorescence microscope sample on the motorized stage while the sample is moving at substantially constant velocity; an objective lens positioned in an optical path between the fluorescence microscope sample on the motorized stage and the TDI line scan camera; an emission filter in the optical path; and at least one processor configured to assemble a plurality of digital images of portions of the fluorescence microscope sample, generated by the TDI line scan camera, into a contiguous digital image of at least a portion of the fluorescence microscope sample.