STABLE AND SOLUBLE ANTIBODIES INHIBITING VEGF

摘要

PROBLEM TO BE SOLVED: To provide stable and soluble anti-VEGF monoclonal antibodies which inhibit vascular endothelial growth factor (VEGF) and is capable of being used for the treatment of solid tumors and various neovascular intraocular diseases.SOLUTION: The present invention relates to soluble and stable anti-VEGF immunobinders comprising CDRs from rabbit monoclonal antibodies. Methods for isolating the hybridomas, nucleic acids, vectors and host cells for expression of the recombinant antibodies of the invention are also disclosed. The present invention provides immunobinders that bind VEGF and thus are suitable to block the function of VEGF in vivo. The CDRs of these immunobinders are derived from rabbit anti-VEGF monoclonal antibodies which were obtained from rabbits that were immunized with human VEGF and/or a fragment thereof.