| 主权项 |
1. A three component molecular biosensor, the molecular biosensor comprising two epitope binding agents constructs and an oligonucleotide construct, which together have formula (III):
R24—R25—R26—R27; R28—R29—R30—R31; O (III)wherein:
R24 is an epitope binding agent that binds to a first epitope on a target antibody; R25 is a flexible linker attaching R24 to R26; R26 and R30 are a pair of nucleotide sequences that are not complementary to each other, but are complementary to two distinct regions on O; R27 and R31 are labels that together comprise a detection means such that when R26 and R30 associate with O a detectable signal is produced, wherein the detection means is selected from the group consisting of fluorescent resonance energy transfer (FRET), lanthamide resonance energy transfer (LRET), fluorescence cross-correlation spectroscopy, flourescence quenching, fluorescence polarization, flow cytometry, scintillation proximity, luminescence resonance energy transfer, direct quenching, chemiluminescence energy transfer, bioluminescence resonance energy transfer, excimer formation, colorimetric substrates detection, phosphorescence, electro-chemical changes, and redox potential changes; R28 is an epitope binding agent that binds to a second epitope on the target antibody; R29 is a flexible linker attaching R28 to R30; and O is a nucleotide sequence comprising a first region that is complementary to R26, and a second region that is complementary to R33. |
