SINGLE CELL-BASED SCREENING METHOD OF USEFUL ENZYME RESOURCES USING MESH-INTEGRATED MICRODROPLET ARRAY

摘要

The present invention relates to a method for manufacturing a picoliter-level microdroplet containing target cells in a single cell unit to analyze the activity of the single cell and to a method for screening interest useful enzyme activity using meshgrid-introduced microdroplet microwell array. The method for manufacturing the microdroplet increases microdroplet production efficiency by secondary crushing when microdroplet is manufactured. Microdroplet microwell array with open-structure using meshgrid method can easily capture and fuse droplets and provide a stable environment to be connected to additional manipulation and another device, which enables that various microorganisms, metagenome-derived metabolism and enzyme activity can be fast searched in the single cell level and is applied to study for interaction between microorganisms in various conditions.