QUANTIFICATION OF VACCINE COMPOSITIONS

摘要

The invention provides methods and mass-labeled peptides for use in said methods for quantifying the presence of a one or more viral proteins in a sample of a preparation containing agents which bind to said viral protein, using mass-spectroscopic analyses of the sample and standards containing known amounts of labeled and unlabeled signature peptides, in particular wherein said viral proteins are antigens in a vaccine for porcine circovirus.

主权项

1. A method of quantifying the presence of a one or more viral proteins in a sample comprising:
a. adding a known quantity of at least one stable-isotope-labeled signature peptide specific to at least one viral protein to the sample; b. digesting the sample with a protease; c. running mass-spectroscopic analyses of the sample; and d. determining the amount of viral protein in the sample.