| 摘要 |
Provided is a DAB-containing substrate kit which can increase specific dyeing intensity and can also prevent the precipitation of DAB in a DAB-containing substrate solution, which is caused by the aggregation of DAB, or the occurrence of non-specific dyeing, such as background dyeing, in a color reaction using a peroxidase-labeled antibody, wherein a color reaction solution containing DAB and imidazole that can act as a sensitizer for DAB is used. The kit according to the present invention comprises (A) a chromophore-containing solution which contains DAB that can act as a peroxidase chromophore and water and (B) a color-developing reagent which comprises at least one chelating agent selected from EDTA·2Na, EDTA·2NH4, GEDTA and NTA, a POE alkyl ether-type nonionic surfactant and/or a POP alkyl ether-type nonionic surfactant, imidazole, hydrogen peroxide and water, and the kit is composed of two or more packs in which at least the chromophore-containing solution (A) and the color-developing reagent (B) are stored separately. |
