PRODUCTION OF NUCLEIC ACID

摘要

PROBLEM TO BE SOLVED: To provide a process for producing nucleic acid encoding a target protein.SOLUTION: The process for the production of nucleic acid encoding a target protein comprises the steps of: (a) providing an array of RNA or DNA molecules including one or more molecules encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bonded to the complex; (c) separating the complexes into compartments in such a manner that most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which activate target protein; (e) selecting nucleic acid encoding the target protein on the basis of the relevant activity; and step (b) is performed in the absence of separate compartments for each complex when the complex is a DNA-protein complex in which the DNA is non-covalently bonded.