| 摘要 |
Microfluidic devices and methods that use cells such as cancer cells, stem cells, blood cells for preprocessing, sorting for various biodiagnostics or therapeutical applications are described. Microfluidics electrical sensing such as measurement of field potential or current and phenomena such as immiscible fluidics, inertial fluidics are used as the basis for cell and molecular processing (e.g., characterizing, sorting, isolation, processing, amplification) of different particles, chemical compositions or biospecies (e.g., different cells, cells containing different substances, different particles, different biochemical compositions, proteins, enzymes etc.). Specifically this invention discloses a few sorting schemes for stem cells, whole blood and circulating tumor cells and also extracting serum from whole blood. Further medical diagnostics technology utilizing high throughput single cell PCR is described using immiscible fluidics couple with single or multi cells trapping technology. |
| 主权项 |
1. A method for sorting electrogenic cells electrically-excitable cells such as neural, cardiac, muscle cells or stem cells by no-stop flow based field potential detection to identify cellular pheotypes using an array of electrodes in the path of the flow of cells which consist of
a. An array of spatially located array electrodes (20 pairs or more) to sense the field potential time signals preceded by a pair of electrodes to stimulate the cells as they flow. b. An electronics system to amplify the field potential signals, filter electrostatic and electromagnetic noises in the system and to reconstruct the time domain signal from the spatially located electrode array c. A sheath flow fluidic system to align the cells with extraction of sheath fluid from diluting the sorted cells followed by a sorting system using pressure fluidic pulse controlled by the detection of iPS cells d. A postprocessing algorithm consists of data processing such as least square fit, baseline correction, subtraction on the reconstructed field potential signals for fluidic control |
