Dual chamber, multi-analyte test strip with opposing electrodes and body fluid test method using this strip

摘要

A dual chamber, multi-analyte test strip (100) has a first insulating layer (102), a first electrically conductive layer (104), with a first working electrode (130), disposed on the first insulating layer (102) and a first patterned spacer layer (108) positioned above the first electrically conductive layer (104). The first patterned spacer layer (108) has a first sample-receiving chamber (134), with first and second end openings (134a,134b), defined therein that overlies the first working electrode (130). The test strip also includes a first counter/reference electrode layer (110) that is exposed to the first sample receiving chamber (134) and is in an opposing relationship to the first working electrode (130). The test strip (100) further includes a counter/reference insulating layer (112) disposed over the first counter/reference electrode layer (110) and a second counter/reference electrode layer (114) disposed on the counter/reference substrate. Also included in the test strip (100) is a second patterned spacer layer (116) that is positioned above the second counter/reference electrode layer (114). The second patterned spacer layer (116) has a second sample-receiving chamber (140), with first and second end openings (140a,140b), defined therein. The test strip (100) additionally has a second electrically conductive layer (120), with a second working electrode (142), disposed above the second patterned spacer layer (116), a second insulating layer (122) disposed above the second electrically conductive layer (120), a first analyte reagent layer (106) disposed on the first working electrode (130) within the first sample-receiving chamber (134); and a second analyte reagent layer (118) disposed on the second working electrode (142) within the second sample-receiving chamber (140). The second counter/reference electrode (114) layer is exposed to the second sample receiving chamber (140) and is in an opposing relationship to the second working electrode (142).