Quil A fraction with low toxicity and use thereof

摘要

Fraction A of Quil A can be used together with at least one other adjuvant for the preparation of an adjuvant composition, where the included adjuvant components act synergistically to enhance level of immune response and have synergistic immunomodulating activity on the co-administered antigens or immunogens.;Other adjuvants can comprise saponins, naturally occurring, synthetic or semisynthetic saponin molecules; e.g. saponins and saponin fractions from Quil A, cell wall skeleton, blockpolymers, TDM, lipopeptides, LPS and LPS-derivatives, Lipid A from different bacterial species and derivatives thereof, e.g., monophosphoryl lipid A. CpG variants, CT and LT or fractions thereof.

主权项

1. A method of enhancement of an immune response level and an immunomodulating activity comprising intraperitoneally or subcutaneously administering to a subject an effective amount of an adjuvant composition with synergistic effect of low toxicity comprising
(i) immunostimulating complex (ISCOM) particles comprising a saponin fraction consisting essentially of fraction A of Quil A, and (ii) at least one other adjuvant, wherein the ISCOM particles comprising the saponin fraction consisting essentially of fraction A of Quil A are less toxic on VERO cells than are QH703 ISCOM matrix particles, and the at least one other adjuvant is in free form or integrated into ISCOM particles other than the ISCOM particles comprising the saponin fraction consisting essentially of fraction A of Quil A.