Method for measuring β-glucan, and β-glucan-binding protein for use in the method

摘要

Disclosed are a method for measuring βG comprising the steps of bringing a sample into contact with a βG-binding protein 1 and a βG-binding protein 2, each comprising an amino acid sequence which is identical or substantially identical to an amino acid sequence shown in any one of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, or SEQ ID NO:20, and having the β-glucan binding activity, to form a complex of the βG-binding protein 1, βG in the sample and the βG-binding protein 2, measuring quantity of the complex, and determining βG concentration in the sample based on the quantity of the complex; a reagent and a kit for use in said method; a novel βG-binding protein; a nucleic acid molecule encoding the βG-binding protein; and a method for producing the aforementioned βG-binding protein.

主权项

1. A method for measuring β-glucan (hereinafter, abbreviated as “βG”), which comprises:
(1) contacting a sample with
protein 1 comprising an amino acid sequence shown in any one of SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, and SEQ ID NO: 10, or an amino acid sequence having 95% or more of homology to the amino acid sequence shown in any one of SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, and SEQ ID NO: 10, and having a β-glucan binding activity (hereinafter, abbreviated as “βG-binding protein 1”), andprotein 2 comprising an amino acid sequence shown in any one of SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, and SEQ ID NO: 10, or an amino acid sequence having 95% or more of homology to the amino acid sequence shown in any one of SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, and SEQ ID NO: 10, having a β-glucan binding activity (hereinafter, abbreviated as “βG-binding protein 2”), to form a complex of the βG-binding protein 1, βG in the sample and the βG-binding protein 2,wherein the βG-binding protein 2 is labeled with a labeling substance; (2) measuring a quantity of the labeling substance in the complex; and (3) determining a βG concentration in the sample based on the quantity measured in (2).