| 主权项 |
1. A method for analysis of at least two analytes in a liquid sample, including a first analyte expected to be in a first concentration and a second analyte expected to be in a second concentration that is much higher than the first concentration, said method comprising the steps of:
providing an analysis device comprising a substrate wherein at least two different types of capturing molecules are immobilized on the substrate in separately distinct areas and wherein each type of capturing molecule has specific affinity for the first and second analytes, respectively, contacting the liquid sample with said immobilized capturing molecules, for the first analyte to be analyzed and expected to have the first concentration: adding at least one type of labeled detection molecule having a specific affinity for the first analyte to be analyzed and inducing contact between the first analyte which is bound to or will be bound to the capturing molecules and the at least one added labelled detection molecule, and for the second analyte to be analyzed and expected to have the second concentration: adding a labeled version or a labeled fragment of the second analyte and inducing contact between the capturing molecules , the second analyte, and the labelled version of the second analyte, and measuring a detectable signal from each of: i) labelled detection molecules, which detection molecules are bound to the first analyte, which first analyte are bound to capturing molecules, which capturing molecules are bound to the substrate and in which the detectable signal increases with an increasing concentration of the first analyte, and ii) the labelled second analyte on the substrate in which the detectable signal decreases with an increasing concentration of the second analyte to be analyzed, wherein the concentration of the labelled analyte is adapted to the concentration of the unlabelled analyte in the liquid sample. |
