| 发明名称 | Method of synthesizing a suppressor tRNA, DNA construct and use thereof for producing a non-natural amino acid-incorporated protein | ||
| 摘要 | There are provided a DNA construct comprising a suppressor tRNA gene of a non-eukaryote containing no internal promoter functioning in a eukaryotic cell, and a eukaryotic or bacteriophage promoter linked at the 5′ end of the tRNA gene, a method for synthesizing a suppressor tRNA by using the DNA construct, and a process for producing protein incorporating a non-natural amino acid by using the same. | ||
| 申请公布号 | US8822180(B2) | 申请公布日期 | 2014.09.02 |
| 申请号 | US201213460773 | 申请日期 | 2012.04.30 |
| 申请人 | Riken | 发明人 | Yokoyama Shigeyuki;Sakamoto Kensaku;Hino Nobumasa;Mukai Takahito;Kobayashi Takatsugu |
| 分类号 | C12N15/11;C12N15/09;C12N15/00 | 主分类号 | C12N15/11 |
| 代理机构 | Birch Stewart Kolasch & Birch, LLP | 代理人 | Birch Stewart Kolasch & Birch, LLP |
| 主权项 | 1. A DNA construct comprising a suppressor tRNA gene derived from Escherichia coli, wherein said tRNA gene contains no internal promoter sequence that functions in a eukaryotic cell, and has a eukaryotic promoter linked to the 5′ end of said tRNA gene wherein the eukaryotic promoter is a pol III type III promoter. | ||
| 地址 | Saitama JP | ||