Light absorbance measurement method and apparatus

摘要

Methods and apparatuses for measuring a light absorbance are provided. The method measures light absorbance of at least one detection chamber of a microfluidic device, including the detection chamber and at least one reference chamber. The detection chamber may accommodate a test subject. The method includes detecting a plurality of reference transmitted light intensities for the at least one reference chamber and estimating a value between the plurality of reference transmitted light intensities through nonlinear approximation. The estimated value is then applied to light absorbance measurement of the detection chamber to reduce a light absorbance error of the detection chamber.

主权项

1. A method to measure light absorbance of at least one detection chamber of a microfluidic device which comprises at least one reference chamber and the at least one detection chamber accommodating a test subject, the method comprising:
detecting a plurality of reference transmitted light intensities for the at least one reference chamber; estimating a value between the plurality of detected reference transmitted light intensities through nonlinear approximation; and applying the estimated value to determine light absorbance of the at least one detection chamber.