Rapid diagnosis of a disease condition using infrared spectroscopy

摘要

Reliable and rapid diagnostic methods for many functional syndromes (FS) such as Bladder Pain Syndrome/Interstitial Cystitis (IC) are not available. Exemplary embodiments include rapid and accurate methods for diagnosing FS in humans and other mammals using infrared microspectroscopy (IRMS). Exemplary methods utilize Soft Independent Modeling by Class Analogy (SIMCA) to create classification models. Exemplary methods utilize classification models to categorize a subject's condition (e.g., healthy/sick and or flare/remission). Using these classification models, various embodiments enable diagnosis based on spectra data from a blood sample or other biomedical specimen. Exemplary embodiments may be useful for rapid diagnosis of IC and various other conditions in humans, cats, and/or other mammals.

主权项

1. A method for rapidly diagnosing a disease condition, comprising:
receiving a fluid biomedical specimen from a test subject; separating the specimen to obtain a desired fraction, wherein the separating removes proteins having a molecular weight greater than 10,000 from the sample; depositing an aliquot of the desired fraction onto a slide; collecting infrared spectral data from the aliquot; and identifying a test subject's condition by analyzing the infrared spectral data using multivariate classification models.