Method for preparing biological tissues for use in biological prostheses

摘要

A method of treating a biological tissue for biological prostheses includes steps of fixation of the biological tissue via a fixing solution including glutaraldehyde and detoxification of the fixed biological tissue via treatment with a detoxifying solution. The detoxification step includes one or both of eliminating phospholipids via treatment with an elimination solution and a treatment with a detoxifying solution. The elimination solution includes 1,2-octanediol and ethanol. The detoxifying solution includes taurine or homocysteic acid.

主权项

1. A method of treating a biological tissue for use in a biological prosthesis, the method comprising the steps:
eliminating phospholipids from the biological tissue via treatment with an elimination solution including 5% v/v 1,2-octanediol and 40% v/v ethanol at a pH of 7.2 and a temperature of 37° C.; fixing the biological tissue via a treatment with a fixing solution containing 0.5% v/v glutaraldehyde in phosphate buffer at a pH of 7.4; and detoxifying the fixed biological tissue via treatment with a detoxifying solution including taurine or homocysteic acid in phosphate buffer at pH 7 and at a temperature above room temperature.