Method for quick and simultaneous determination of 16 inorganic anions and organic acids in tobacco

摘要

The present invention discloses a method for quick and simultaneous determination of 16 inorganic anions and organic acids in tobacco by ion chromatography The retention behavior of inorganic anions and organic acids on the anion exchange column was investigated using potassium hydroxide produced by EGC-II KOH eluent autogenerator as eluent. The optimized gradient elution condition was obtained. The samples were prepared through extraction, filtration and dilution before analysis. The separation was performed on an anion exchange column. The time of the gradient elution program was 50 mins. Under the optimized conditions, the calibration of peak area for all the analytes were linear in the ranges of 105. The method in the present invention has the advantages of simplicity, rapidity and accuracy, and is able to simultaneously determine 16 inorganic anions and organic acids in tobacco by one single time of injection.

主权项

1. A method for quick and simultaneous determination of 16 inorganic anions and organic acids in tobacco by ion chromatography, wherein the elution process comprises:
analysing filtrates extracted from a tobacco sample in accordance with the following elution process and conditions;Concentration Timeof KOH(min)(ρ/mmol · L−1)CurveDescription0.00015Start of analysis8.00015Analysis of weak retention components28.000305Analysis of medium retentioncomponents35.000505Analysis of strong retention components40.000505Purge system40.00115Balance system50.00015End of analysis conditions of said ion chromatograghy being: anion exchange column: IonPacAS11-HC (2 mm×50 mm) and IonPacAG11-HC (2 mm×250 mm); column temperature: 30° C.; flow rate: 0.30 ml/min; injection volume: 25 μL; suppressor current: 50 mA; and wherein the method further comprises steps for pre-treating the tobacco sample before analysis, the steps for pre-treating the tobacco sample consisting of: (1) determining the percentage of moisture in the tobacco sample; (2) pre-treating the tobacco sample by extracting the tobacco sample ultrasonically by extraultra-purified water at a ratio of 1 g tobacco sample: 10 mL water and then filtering the sample to collect the filtrates; and (3) diluting the filtrates tenfold and then filtering the filtrates.