Method of generating natural killer cells and dendritic cells from human embryonic stem cell-derived hemangioblasts

摘要

This invention provides methods of generating natural killer (NK) cells and dendritic cells (DCs). The methods utilize human hemangioblasts as intermediate cells to generate the NK cells and DCs. In various embodiments, the methods do not require the use of stromal feeder layers.

主权项

1. A method to generate dendritic cells (DCs), comprising:
providing hemangioblasts; culturing the hemangioblasts in liquid media comprising human serum, SCF, FL, IL3 and GM-CSF; adding IL4 to the liquid media; and further culturing the hemangioblasts to generate the DCs.